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Antiviral Resistance in Plants
Buch
Buch
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Fachbuch
2012

Antiviral Resistance in Plants

Methods and Protocols

Kollektion

Methods in Molecular Biology

ISBN
EAN
978-1-61779-881-8
9781617798818
Artikel-Nr.
PR474GZ
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3
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Rabatt
-14.6
%
CHF 197.00
CHF
168.22
Anzahl
1
Maximale
Lieferzeit
5
Arbeitstage
Donnerstag
05.08.2021
speech-bubble-svg Beschreibung

Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In Antiviral Resistance in Plants: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.  These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols seeks to aid scientists in the further study of this crucially important botanical trait.
Inhaltsverzeichnis
1. An  Historical Overview of RNAi in Plants            John A. Lindbo 2. RNA Silencing and Antiviral Defense in Plants            Claire Agius, Andrew Eamens, Tony Millar, John M. Watson, and Ming-Bo Wang 3. Biological Chemistry of Virus-encoded Suppressors of RNA Silencing:  An Overview            Rustem T. Omarov and Herman B. Scholtof 4. Virus-Induced Gene Silencing Using Begomovirus Satellite Molecules            Xueping Zhou and Changjun Huang 5. Strategies for Viral Cross Protection in Plants            Changyong Zhou and Yan Zhou 6. Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing            Shawn Bachan and S.P. Dinesh-Kumar 7. Virus-Induced Gene Silencing in Hexaploid Wheat Using Barley Stripe Mosaic Virus Vectors            Steven R. Scofield and Amanda S. Brandt 8. Characterization of Plant Virus-Encoded Gene Silencing Suppressors            Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing            Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants            Robert S. Anderssen and Peter M. Waterhouse 11. Isolation and Detection of Small RNAs from Plant Tissues            Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants            Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance            Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Hajime Yaegashi, Masamichi Isogai, and Nobuyuki Yoshikawa 9. Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing            Qian-Hao Zhu and Chris Helliwell 10. Modelling Antiviral Resistance in Plants            Robert S. Anderssen and Peter M. Waterhouse 11. Isolation and Detection of Small RNAs from Plant Tissues            Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants            Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance            Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Neil A. Smith and Andrew L. Eamens 12. Isolation and Analysis of Small RNAs from Virus-Infected Plants            Shaun J. Curtin, Agustin Zsögön, John M. Watson, and Peter M. Waterhouse 13. Use of Hairpin RNA Constructs for Engineering Plant Virus Resistance            Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Neena Mitter and Ralf G. Dietzgen 14. Artificial MicroRNAs for Plant Virus Resistance            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Jing Qu, Jian Ye, and Rongxiang Fang 15. Isolation and Biochemical Analysis of Plant Small RNAs            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            H. Alexander Ebhardt, Mariana Oviedo Ovando, and Peter J. Unrau 16. Isolation and Analysis of Plant RNA-Dependent RNA Polymerases            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Julien Curaba 17. Plant Viroids: Isolation, Characterization/Detection, and Analysis            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Robert A. Owens, Teruo Sano,  and Nuria Duran-Vila 18. Structural and Functional Analysis of CMV Satellite RNAs in RNA Silencing            Hanako Shimura and Chikara Masuta 19. Virus-Induced Gene Silencing in Soybean            Aardra Kachroo and Said Ghabrial 20. Chimeric, Infectious, and Stable Virus Transcripts to Study RNA Silencing in ‘Dark Green’ Islands            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy            Robin M. MacDiarmid 21. Rolling Circle Amplification-Mediated Hairpin RNA (RMHR) Library Construction in Plants            Lei Wang and Yun-Liu Fan 22. The Interaction Between Plant Viroid-Induced Symptoms and RNA Silencing            Vicente Pallas, German Martinez, and Gustavo Gomez 23. Identification of Small Molecule Inhibitors of Tomato Bushy Stunt Virus (TBSV) Replication            Zsuzsanna Sasvari and Peter D. Nagy 24. Expression of Dominant-Negative Mutants to Study Host Factors Affecting Plant Virus Infections            Daniel Barajas and Peter D. Nagy
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Vereinigte Staaten
12.06.2012
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Englisch
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Hardcover
380 Seiten
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