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Microbial Carotenoids from Bacteria and Microalgae
Buch
Buch
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Fachbuch
2012

Microbial Carotenoids from Bacteria and Microalgae

Methods and Protocols

Kollektion

Methods in Molecular Biology

ISBN
EAN
978-1-61779-878-8
9781617798788
Artikel-Nr.
LVK4KGE
Sofort lieferbar:
2
Kostenloser Versand
Rabatt
-14.5
%
CHF 193.00
CHF
164.94
Anzahl
1
Maximale
Lieferzeit
5
Arbeitstage
Donnerstag
05.08.2021
speech-bubble-svg Beschreibung

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activity are described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic Dunaliella species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in Anabaena sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.

Inhaltsverzeichnis
1. Pathways of Carotenoids Biosynthesis in Bacteria and MicroalgaeJ. Paniagua-Michel, Jorge Olmos-Soto, Manuel Acosta Ruiz 2. Selection and Taxonomic Identification of Carotenoids Producing Marine Actinomycetes Francisco Romero, Rosa Isabel Fernández-Chimeno, Juan Luis de la Fuente, and José Luis Barredo 3. Isolation, Characterization and Diversity of Novel Radio-tolerant Carotenoids-producing Bacteria Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 4. A Novel Radio-tolerant Astaxanthin Producing Bacterium Reveals a New Astaxanthin Derivative: Astaxanthin Dirhamnoside Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coliHisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin BiosynthesisQiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaeaPatricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  4. A Novel Radio-tolerant Astaxanthin Producing Bacterium Reveals a New Astaxanthin Derivative: Astaxanthin Dirhamnoside Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coliHisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin BiosynthesisQiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaeaPatricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  5. Novel Zeaxanthin Producing Bacteria Isolated from a Radioactive Hot Spring Water Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda 6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coliHisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin BiosynthesisQiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaeaPatricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  6. Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coliHisashi Harada and Norihiko Misawa 7. Engineering Escherichia coli for Canthaxanthin and Astaxanthin BiosynthesisQiong Cheng, and Luan Tao 8. Analysis of Canthaxanthin Production by Gordonia jacobaeaPatricia Veiga-Crespo, Teresa Vinuesa, Miquel Viñas, and Tomás G. Villa 9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  9. Isolation and Light-stimulated Expression of Canthaxanthin and Spirilloxathin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278Eric Giraud, and André Verméglio 10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  10. Construction of Carotenoid Biosynthetic Pathways Through Chromosomal Integration in Methane-Utilizing Bacterium Methylomonas sp. Strain 16aRick W. Ye and Kristen Kelly 11. Genetic Modification in Bacillus subtilis for Production of C30 CarotenoidsIsamu Maeda 12. Carotenoids Production from Halophilic BacteriaMaría de Lourdes Moreno, Cristina Sánchez-Porro, María Teresa García and Encarnación Mellado 13. Construction and Utilization of Carotenoid Reporter Systems: Identification of Chromosomal Integration Sites that Support Suitable Expression of Biosynthetic Genes and PathwaysPamela L. Sharpe and Deana J. DiCosimo14. Directed Evolution of Carotenoid Synthases for the Production of Unnatural CarotenoidsMaiko Furubayashi and Daisuke Umeno 15. A High Throughput Screen for the Identification of Improved Catalytic activity: b-carotene HydroxylaseMark A. Scaife, Cynthia A. Ma, Phillip C. Wright, and Roberto E. Armenta 16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  16. DNA Fingerprinting Intron-sizing Method to Accomplish a Specific,Rapid and Sensitive Identification of Carotenogenic Dunaliella SpeciesJorge Olmos, J. Paniagua-Michel, Rosalia Contreras and Leonel Ochoa 17. Ketocarotenoid Biosynthesis in Transgenic Microalgae Expressing a Foreign β-C-4-carotene Oxygenase GeneMarta Vila, Aurora Galván, Emilio Fernández, Rosa León 18. Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi 19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser  19. Obtaining Lutein Rich Extract from Microalgal Biomass at Preparative ScaleJosé M. Fernández-Sevilla, F. Gabriel Acién Fernández and Emilio Molina Grima 20. NMR-based Isotopologue Profiling of Microbial CarotenoidsEva Eylert, Adelbert Bacher and Wolfgang Eisenreich 21. Analysis of Diapocarotenoids Found in Pigmented Bacillus SpeciesLaura Pérez-Fons, and Paul D. Fraser
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23.05.2012
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Englisch
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355 Seiten
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